Last edited by Dokree
Wednesday, August 5, 2020 | History

6 edition of Rapid freezing, freeze fracture, and deep etching found in the catalog.

Rapid freezing, freeze fracture, and deep etching

  • 111 Want to read
  • 20 Currently reading

Published by Wiley-Liss in New York .
Written in English

    Subjects:
  • Freeze fracturing.,
  • Freeze-etching.,
  • Freeze Fracturing -- methods.,
  • Microscopy, Electron -- methods.,
  • Freeze Etching -- methods.,
  • Freezing.,
  • Cell Membrane -- ultrastructure.

  • Edition Notes

    Includes bibliographical references and index.

    Statementedited by Nicholas J. Severs and David M. Shotton.
    SeriesTechniques in modern biomedical microscopy
    ContributionsSevers, Nicholas J., Shotton, David.
    Classifications
    LC ClassificationsQH236.2 .R36 1995
    The Physical Object
    Paginationxi, 372 p. :
    Number of Pages372
    ID Numbers
    Open LibraryOL1277426M
    ISBN 100471014338
    LC Control Number95009125

    () Rapid Freezing, Freeze Fracture and Deep Etching: Techniques in Modern Biomedical Microscopy, Wiley-Liss, New York, NY Rochow, T.G. and P.A. Tucker, Introduction to Microscopy by Means of Light, Electrons, X-Rays, or Acoustics Second Ed., Plenum Press, New York, NY. Severs NJ. Freeze-fracture cytochemistry: an explanatory survey of methods. In “Rapid Freezing, Freeze Fracture, and Deep Etching” (NJ Severs, DM Shotton eds.), , pp. – Wiley-Liss, New York. Google Scholar.

    DeepDyve is the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. To produce higher-resolution images of the tip-link fine structure, we used the rapid-freeze, deep-etch technique (11, 12), with improved metal replication and imaging procedures. (Using the quick-freeze, deep-etch technique, we have analyzed the structure of the intact cell wall of Chlamydomonas reinhardi, and have visualized its component glycoproteins after mechanical shearing and after.

    CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): Abstract. Physical fixation by rapid freezing followed by freeze-fracture and deep-etching has provided the means for potentially seeing the three-dimensional arrangement in the native state of particles on mitochondrial inner membranes. We have used these techniques to study the tubular cristae of Paramecium in the.   A high vacuum low temperature atomic force microscope (AFM) for the direct observation of biological freeze–fracture samples has been developed. This AFM has an integrated vacuum system and a freeze–fracture mechanism inside the vacuum chamber. It is possible to observe the fractured sample surface without exposing the freshly fractured surface to the ambient atmosphere.


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Rapid freezing, freeze fracture, and deep etching Download PDF EPUB FB2

Rapid Freezing, Freeze Fracture, and Deep Etching guides the reader through the principles of these techniques and gives detailed examples of their application in biomedical research.

The book is organized into three sections. The final chapters offer a series of applications selected to illustrate freeze fracture wide-ranging and enduring impact of rapid freezing, freeze fracture, and deep etching on the field of biomedical.

An introduction to freeze fracture and deep etching / David M. Shotton and Nicholas J. Severs --A practical introduction to rapid freezing techniques / Nicholas J.

Severs, Terence M. Newman, and David M. Shotton --A guide to equipment for production of freeze-fracture replicas / Terence M. Newman --Freeze-fracture artifacts: freeze fracture to recognize and avoid them / Rukmal M. Abeysekera and Anthony. Newman TM () A guide to equipment for production of freeze-fracture replicas.

In: Severs NJ, Shotton DM (eds) Rapid freezing, freeze fracture and deep etching. Wiley-Liss Inc., New York, pp 51–67 Google ScholarAuthor: Douglas E. Chandler, William P.

Sharp. C H A P T E R 25 Rapid Freezing of Biological Specimens for Freeze Fracture and Deep Etching Nicholas J. Severs and David M. Shotton I. PRINCIPLES OF RAPID FREEZING Stabilization of biological structure by the physical process of freezing (cryofixation) forms the starting point for freeze fracture and deep etching (see article by Shotton) and for freeze substitution, cryoultrami- crotomy, Cited by: 3.

For further information on the theory and practice of freeze fracture and related techniques, the reader is referred to the standard reference book on and deep etching book topic, Rapid Freezing, Freeze Fracture.

New Releases Rapid Freezing, Freeze Fracture and Deep Etching (Techniques in Modern Biomedical. Tools Physical fixation by rapid freezing followed by freeze-fracture and deep-etching has provided the means for potentially seeing the three-dimensional arrangement in the native state of particles on mitochondrial inner membranes.

Freeze-etching technique combined with a simple rapid freezing method is described. The freezing of tissues was accomplished by bringing them into contact with cold copper block at the temperature of liquid nitrogen. The frozen tissues were put into deep grooves on.

5 Bert Ph. Menco, A survey of ultra-rapid cryofixation methods with particular emphasis on applications to freeze-fracturing, freeze-etching, and freeze-substitution, Journal of Electron Microscopy Technique,4, 3, Wiley Online Library.

In "Rapid Freezing, Freeze Fracture and Deep Etching" (N. Severs and D. Shotton, eds.), pp. Wiley-Liss, New York. Wiley-Liss, New York. Branton, D., Bullivant, S., Gilula, N.

B., Karnovsky, M. J., Moor, H., Mühlethaler, K., Northcote, D. H., Packer, L., Satir, B., Satir, P., Speth, V., Staehlin, L. A., Steere, R. L., and Weinstein, R. PRINCIPLES OF RAPID FREEZING Stabilization of biological structure by the physical process of freezing (cryofixation) forms the starting point for freeze fracture and deep etching (see article by Shotton) and for freeze substitution, cryoultramicrotomy, and cryoelectron microscopy (see articles by Roos et al., and Resch et al.).

Severs, N. and Shotton, D. M., ed. () Rapid Freezing, Freeze Fracture, and Deep Etching. Wiley-Liss, New York.

Google Scholar. T1 - Freeze fracture and freeze etching. AU - Chandler, Douglas E. AU - Sharp, William P. PY - Y1 - N2 - Freeze fracture depends on the property of frozen tissues or cells, when cracked open, to split along the hydrophobic interior of membranes, thus revealing broad panoramas of.

Freeze-fracture, deep etching of hair cells reveals the structural elements in and around stereocilia and in the apical cytoplasm of the hair cells, and their relationship to plasma membrane structures, exposed by fracture.

The actin filaments of the stereocilia and extracellular crosslinks between adjacent stereocilia are exposed (fig. 2a). A technique borrowed from biology, rapid cryofixation/freeze fracture, has been adapted for the study of liquid–solid interfaces. This technique allows high-resolution imaging of the interfaces between water and substrates with varying degrees of hydrophobicity.

An overview of the evolution of freeze fracture and freeze etching techniques has been prepared recently by Heuser. Rapid freezing and freeze fracture. Ultra rapid freezing of specimen by plunging, spraying, cold metal block contact, or high pressure freezing.

Deep etching and freeze drying of cell cortices and macromolecules. Bert Ph. Menco, A survey of ultra‐rapid cryofixation methods with particular emphasis on applications to freeze‐fracturing, freeze‐etching, and freeze‐substitution, Journal of Electron Microscopy Technique, /jemt, 4, 3, (), ().

There are four essential steps in making a freeze-fracture replica: (i) rapid freezing of the specimen, (ii) fracturing the specimen, Brief or deep etching, or complete freeze-drying, may also be. Specimens were deciliated in preparation for the production of surface replicas by rapid agitation (2min) in 5% (v/v) ethanol.

The deciliated cells were then spread over single mica strips and frozen in freshly melted Freon The strips were loaded onto the monolayer freeze-fracture device and placed in the freeze-fracture plant for deep-etching.

cells using a procedure for rapid freezing before freeze- fracture and deep-etching A. FORGE 1., S. DAVIES 1 and G. ZAJIC 2 1 Structural Biology Laboratory, and Department of Audiology, Institute of Laryngology and Otology, University College London, Gray' s Inn Road, London WC1X 8EE, UK.Freeze etching.

English. English Español Português Français Italiano Svenska Deutsch. Fractures, Bone Hip Fractures Femoral Fractures Spinal Fractures. Chemicals and Drugs 8.

Tetrathionic Acid Glycoproteins Membrane Lipids Connexins Connexin 43. Abstract Physical fixation by rapid freezing followed by freeze-fracture and deep-etching has provided the means for potentially seeing the three- dimensional arrangement in the native state of particles on mitochondrial inner membranes.